The Potential of 2-aza-8-Oxohypoxanthine as a Cosmetic Ingredient

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal keratinocyte (NHEK) cells. Gene expression levels were determined analysis 177 genes involved skin aging and disease. AOH showed a significant increase viability at concentrations between 7.8 31.3 μg/mL decrease above 250 μg/mL. that significantly increased gene CLDN1, DSC1, DSG1, CDH1 (E-cadherin), are intercellular adhesion barrier functioning. also up-regulated KLK5, KLK7, SPIMK5, proteases stratum corneum detachment. Furthermore, stimulated KRT1, KRT10, TGM1, IVL, considered general differentiation indicators, SPRR1B, cornified envelope component protein. exerted activation effect Since did not cause cytotoxicity, it compound had no adverse skin. addition, found functioning Therefore, has potential for practical use as cosmetic ingredient. This is first report efficacy evaluation tests performed AOH.